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JoVE Journal Developmental Biology
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
 

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Article DOI: 10.3791/56919-v • 05:49 min • January 2nd, 2018
January 2nd, 2018 •
1, 1,2
1Department of Molecular and Human Genetics, Baylor College of Medicine, 2Program in Developmental Biology, Baylor College of Medicine

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Summary
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Complexity of in vivo systems makes it difficult to distinguish between the activation and inhibition of Notch receptor by trans- and cis-ligands, respectively. Here, we present a protocol based on in vitro cell-aggregation assays for qualitative and semi-quantitative evaluation of the binding of Drosophila Notch to trans-ligands vs cis-ligands.

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Keywords: Cell Aggregation Assay Notch Receptor Trans-ligands Cis-ligands Drosophila S2 Cells Ligand Binding Cell Signaling Aggregation Quantification Microscopy Hemacytometer
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