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Journal / Bioengineering / Preparation, Purification, and Use of Fatty…
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Bioengineering

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JoVE Journal Bioengineering
Preparation, Purification, and Use of Fatty Acid-containing Liposomes
 

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Article DOI: 10.3791/57324-v • 10:43 min • February 9th, 2018
February 9th, 2018 •
*1,2, *1,3, 1,3, 1
1Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 2Department of Biomedical Engineering, Boston University, 3Department of Genetics, Cell Biology, and Development, University of Minnesota
* These authors contributed equally

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Summary
Automatic Translation

Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these amphiphiles.

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Keywords: Fatty Acid Liposomes Vesicles Synthetic Biology Origin Of Life Minimal Cells Artificial Protocells Thin Film Rehydration Extrusion Polycarbonate Membrane Tris-HCL Buffer
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