Journal
/
Cancer Research
/
Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts

Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts

DOI:
10.3791/57448-v

•

07:52 min

•

April 26, 2018

•

, , , ,
1Ludwig Institute for Cancer Research, 2Moores Cancer Center, School of Medicine,University of California, San Diego, 3Department of Pathology, School of Medicine,University of California, San Diego, 4Department of Medicine, School of Medicine,University of California, San Diego

Chapters

  • 00:05Title
  • 00:55Construct-positive Glioblastoma Cell Sorting
  • 02:46Near-infrared Fluorescent Protein (iRFP)-tagged Glioblastoma Cell Intracranial Injection
  • 04:49Fluorescence Molecular Tomography (FMT) Imaging and Analysis
  • 06:22Results: Representative In Vivo FMT Imaging of Glioblastoma Xenografts
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

Orthotopic intracranial injection of tumor cells has been used in cancer research to study brain tumor biology, progression, evolution, and therapeutic response. Here we present fluorescence molecular tomography of tumor xenografts, which provides real-time intravital imaging and quantification of a tumor mass in preclinical glioblastoma models.

Tags

Keywords: Fluorescence Molecular TomographyFMTGlioblastoma XenograftsIn Vivo ImagingBrain Tumor BiologyCancer ResearchDrug DiscoveryTumor AggressivenessTumor HeterogeneityTreatment ResponsivenessNon-invasiveQuantitativeXenograft ModelsGlioblastoma CellsLentivirusFluorescent ProteinFlow CytometryCell SortingSingle Cell SuspensionPBSIn Vivo Experiments

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57448?article_type=v

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
check_url/57448?article_type=v

A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines
check_url/57448?article_type=v

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
check_url/57448?article_type=v

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
check_url/57448?article_type=v

Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice
check_url/57448?article_type=v

In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research
check_url/57448?article_type=v

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
check_url/57448?article_type=v

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
check_url/57448?article_type=v

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
check_url/57448?article_type=v

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Read Article