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Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection
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Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection

Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection

DOI:
10.3791/57628-v

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08:50 min

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May 13, 2018

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, ,
1Environmental and Life Sciences Graduate Department,Trent University, 2Forensic Science Department,Trent University

Chapters

  • 00:04TItle
  • 01:01Induction of Germination for Respiration Monitoring and Obtaining Oxygen Consumption Rate (OCR) Measurements
  • 02:34Induction of Teliospore Germination to Isolate Teliospores at Distinct Stages of Germination and Preparation of Micromanipulator
  • 04:34Isolation of Stage-specific Germinating Teliospores
  • 06:01Results: Microrespiration Analysis and Microdissection for Assessing Teliospore Germination
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Smut fungi cause many devastating agricultural diseases. They are dispersed as dormant teliospores that germinate in response to environmental cues. We outline two methods to investigate molecular changes during germination: measuring respiration increase to detect metabolic activation and assessing changing molecular events by isolating teliospores at distinct morphological stages.

Tags

Keywords: Teliospore GerminationMicrorespiration AnalysisMicrodissectionRespiration LevelGene Transcript LevelsPathogen DisseminationCorncob InfectionTeliospore ViabilityTeliospore SuspensionOxygen Consumption Rate (OCR)Potato Dextrose BrothStreptomycin SulfateKanamycin SulfateErlenmeyer Flask

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