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A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
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Cancer Research
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JoVE Journal Cancer Research
A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager

DOI:
10.3791/59042-v

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09:17 min

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February 02, 2019

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, , ,
1Medical Genetics,Hunter Medical Research Institute, 2Priority Research Centre for Cancer Research, Innovation and Translation, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine,University of Newcastle, 3Pathology North,John Hunter Hospital

Chapters

  • 00:04Title
  • 00:51Optimal Migration Assay Seeding Density
  • 02:49Migration and Invasion Assay Preparation
  • 03:36Wound Scratch
  • 05:35Wound Assay Imaging and Data Analysis
  • 06:43Results: Representative Migration and Invasion Assay Analyses
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

The current protocol describes an integrated method investigating cancer cell migration and invasion on a single platform in real-time, providing an easily reproducible and time-efficient option to study cell mobility and morphology.

Tags

Keywords: Cell MigrationCell InvasionCancer Cell MobilityMetastasisLive-cell ImagerAutomated ImagingCell ProliferationExtracellular MatrixScratch AssayCell Density OptimizationReal-time Analysis

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