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Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
This content is Free Access.
1Molecular Biology Interdepartmental Program,University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology,University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine,University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center,University of California, Los Angeles, 6Molecular Biology Institute,University of California, Los Angeles
Chapters
- 00:05Title
- 00:52Plating Sorted Prostate Epithelial Cells into Primary Mouse Organoid Culture
- 03:29Extracting Protein Lysate from Prostate Organoids for Western Blot Analysis
- 04:50Fixing and Staining Prostate Organoids for Immunohistochemical Analysis by Whole-Mount Confocal Microscopy
- 06:44Tissue Clearing and Mounting of the Stained Prostate Organoids for Whole-mount Confocal Microscopy
- 07:57Results: Analysis of Lineage Marker Expression in Prostate Organoids by Western Blot and Whole-Mount Confocal Microscopy
- 09:44Conclusion
Mouse prostate organoids represent a promising context to evaluate mechanisms that regulate differentiation. This paper describes an improved approach to establish prostate organoids, and introduces methods to (1) collect protein lysate from organoids, and (2) fix and stain organoids for whole-mount confocal microscopy.
