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Developmental Biology

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JoVE Journal Developmental Biology
Isolamento e la coltura di Neurosfere Zebrafish adulti Brain-derived
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Isolamento e la coltura di Neurosfere Zebrafish adulti Brain-derived

Article DOI: 10.3791/53617-v • 11:01 min • February 29th, 2016
February 29th, 2016 •
*1,2, *3, 1, 1,3, 1
1Yale Cardiovascular Research Center, Internal Medicine, Yale University, 2Department of Medicine, University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière, Université Pierre and Marie Curie
* These authors contributed equally

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Qui forniamo un metodo riproducibile per esaminare neurogenesi adulta usando un test neurosfere derivato dal cervello intero o sia dal telencefalica, tectal o nelle regioni del cervelletto del cervello zebrafish adulto. Inoltre, si descrive la procedura per manipolare l'espressione genica in neurosfere zebrafish.

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Biologia dello Sviluppo Numero 108 Zebrafish neurogenesi neurali adulte cellule staminali / progenitrici saggio neurosfere miRNA la manipolazione genetica
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