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Journal / Immunology and Infection / iDISCO+およびライトシート蛍光顕微鏡による椎体リンパ管系および排水の3次元画像化…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
iDISCO+およびライトシート蛍光顕微鏡による椎体リンパ管系および排水の3次元画像化
Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment
Journal (Immunology and Infection)
Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
Journal (Immunology and Infection)
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
 
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iDISCO+およびライトシート蛍光顕微鏡による椎体リンパ管系および排水の3次元画像化

Article DOI: 10.3791/61099-v • 10:05 min • May 22nd, 2020
May 22nd, 2020 •
*1, *1,2, 1,3
1Université Pierre et Marie Curie Paris, Sorbonne Université, Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 3Department of Neurology, Yale University School of Medicine
* These authors contributed equally

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組織クリアリングと光シート蛍光顕微鏡(LSFM)を組み合わせて、脳脊髄液(CSF)および脊髄硬膜外液を収集するリンパ管およびリンパ節(LN)の3次元および細胞分解能画像を得るプロトコルが提示される。

Tags

免疫学と感染症、問題159、髄膜リンパ管、リンパ節、排液、iDISCO+、ライトシート蛍光顕微鏡、中枢神経系
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