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Journal / Neuroscience / Multiplex Fluorescence In Situ Hybridization with …
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Neuroscience

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JoVE Journal Neuroscience
Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Section(신선 냉동 또는 고정 마우스 뇌 절편에서 Multiplex Fluorescence In Situ Hybridization과 형광 면역조직화학의 결합)
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
Journal (Neuroscience)
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes
Journal (Neuroscience)
Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes
 
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Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Section(신선 냉동 또는 고정 마우스 뇌 절편에서 Multiplex Fluorescence In Situ Hybridization과 형광 면역조직화학의 결합)

Article DOI: 10.3791/61709-v • 07:36 min • June 25th, 2021
June 25th, 2021 •
1, 1, 1
1Department of Pharmacology, School of Medical Sciences, University of New South Wales

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이 프로토콜은 다중 표지 FISH 및 형광 IHC 신호를 달성하는 것을 목표로 신선 냉동 및 고정 마우스 뇌 절편 모두에서 형광 in situ hybridization(FISH)과 형광 면역조직화학(IHC)을 결합하는 방법을 설명합니다. IHC는 세포질 및 막 부착 단백질을 표적으로 삼았습니다.

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다중 형광 In Situ Hybridization FISH 형광 면역조직화학 신선 냉동 마우스 뇌 절편 고정 마우스 뇌 절편 RNA 전사체 신경화학적 표현형 항체 단백질 라벨링 RNAscope FISH 형광 면역염색 Galanin Receptor 1 Glycine Transporter 2 뇌간 핵
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