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JoVE Journal Neuroscience
Kombinera multiplex fluorescens in situ-hybridisering med fluorescerande immunhistokemi på färskfrysta eller fixerade mushjärnsnitt
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Kombinera multiplex fluorescens in situ-hybridisering med fluorescerande immunhistokemi på färskfrysta eller fixerade mushjärnsnitt

Article DOI: 10.3791/61709-v • 07:36 min • June 25th, 2021
June 25th, 2021 •
1, 1, 1
1Department of Pharmacology, School of Medical Sciences, University of New South Wales

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Detta protokoll beskriver en metod för att kombinera fluorescens in situ-hybridisering (FISH) och fluorescensimmunhistokemi (IHC) i både färskfrysta och fixerade mushjärnsnitt, med målet att uppnå multilabel FISH och fluorescens IHC-signal. IHC-riktade cytoplasmatiska och membranbundna proteiner.

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Multiplex fluorescens in situ-hybridisering FISH fluorescerande immunhistokemi färskfrysta mushjärnsnitt fixerade mushjärnsnitt RNA-transkript neurokemisk fenotypning antikroppar proteinmärkning RNAscope FISH fluorescensimmunfärgning galaninreceptor 1 glycintransportör 2 hjärnstamskärnor
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