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Journal / Immunology and Infection / Untersuchung der Aortenklappenverkalkung durch…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Untersuchung der Aortenklappenverkalkung durch Isolierung und Kultur von T-Lymphozyten unter Verwendung von Feederzellen aus bestrahltem Buffy-Fell
Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
Journal (Immunology and Infection)
Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface (Video) | JoVE
Journal (Immunology and Infection)
Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface (Video) | JoVE
 
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Untersuchung der Aortenklappenverkalkung durch Isolierung und Kultur von T-Lymphozyten unter Verwendung von Feederzellen aus bestrahltem Buffy-Fell

Article DOI: 10.3791/62059-v • 04:30 min • February 4th, 2021
February 4th, 2021 •
*1,2, *1, 3,4, 1,3, *2,5, *1,6, *1,6
1Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine, University of Florence, 3Berlin Institute of Health, 4Department of Cardiology, German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine, Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology, Helios Klinikum Erfurt
* These authors contributed equally

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In dieser Studie beschreiben wir den Prozess der T-Lymphozyten-Isolierung aus frischen Proben verkalkter Aortenklappen und die analytischen Schritte des T-Zell-Klonens zur Charakterisierung der adaptiven Leukozyten-Untergruppen unter Verwendung der Durchflusszytometrie-Analyse.

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Immunologie und Infektion Ausgabe 168 Aortenklappenerkrankung Aortenstenose T-Zell-Extraktion TAVI Buffy Coat Durchflusszytometrie-Analyse adaptive Immunität.
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