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Journal / Biochemistry / Nauwkeurige en nauwkeurige FRET-metingen met één…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Nauwkeurige en nauwkeurige FRET-metingen met één molecuul uitvoeren met behulp van de Open-Source smfBox
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Nauwkeurige en nauwkeurige FRET-metingen met één molecuul uitvoeren met behulp van de Open-Source smfBox

Article DOI: 10.3791/62378-v • 07:12 min • July 5th, 2021
July 5th, 2021 •
*1, *1, 1, 1
1Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
* These authors contributed equally

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Dit artikel biedt stapsgewijze instructies voor het uitvoeren van volledig gecorrigeerde nauwkeurige FRET-metingen op individuele, vrij verspreidende biomoleculen met behulp van de open-source, goedkope smfBox, van inschakelen, via uitlijning en scherpstellen, tot gegevensverzameling en -analyse.

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Biochemie Nummer 173 enkel molecuul FRET microscopie fluorescentie DNA biomoleculaire conformatie confocaal structuurbepaling dynamica
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