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Journal / Bioengineering / Mikroinjeksjon av maisplanthopper, Peregrinus…
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Bioengineering

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JoVE Journal Bioengineering
Mikroinjeksjon av maisplanthopper, Peregrinus maidis, embryoer for CRISPR/Cas9-genomredigering
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Mikroinjeksjon av maisplanthopper, Peregrinus maidis, embryoer for CRISPR/Cas9-genomredigering

Article DOI: 10.3791/62417-v • 07:27 min • March 26th, 2021
March 26th, 2021 •
*1, *1, 1, 1, 1, 1, 1
1Department of Entomology and Plant Pathology, North Carolina State University
* These authors contributed equally

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Her er protokoller for innsamling og mikroinjeksjon av precellulære maisplantehopperembryoer med det formål å modifisere genomet deres via CRISPR / Cas9-basert genomredigering eller for tilsetning av markerte transponerbare elementer gjennom kimlinjetransformasjon.

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Bioteknologi utgave 169 embryonal mikroinjeksjon funksjonell genomikk kimlinjetransformasjon CRISPR/Cas9 genomredigering maisplanthopper Hemiptera
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