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Journal / Bioengineering / Molekulare Federkonstantenanalyse mittels…
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Bioengineering
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JoVE Journal Bioengineering
Molekulare Federkonstantenanalyse mittels Biomembran-Kraftsondenspektroskopie
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Molekulare Federkonstantenanalyse mittels Biomembran-Kraftsondenspektroskopie

Article DOI: 10.3791/62490-v • 08:10 min • November 20th, 2021
November 20th, 2021 •
1, 1,2,3, 1, 1,4, 1, 5, 5, 1,2, 1,2,3
1School of Biomedical Engineering, Faculty of Engineering, The University of Sydney, 2Charles Perkins Centre, The University of Sydney, 3Heart Research Institute, 4Department of Chemistry, The Hong Kong University of Science and Technology, 5School of Aerospace, Mechanical and Mechatronic Engineering, Faculty of Engineering, The University of Sydney

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Eine Biomembrankraftsonde (BFP) ist eine In-situ-Technik der dynamischen Kraftspektroskopie (DFS). BFP kann verwendet werden, um die Federkonstante molekularer Wechselwirkungen auf lebenden Zellen zu messen. Dieses Protokoll präsentiert eine Federkonstantenanalyse für molekulare Bindungen, die von BFP nachgewiesen wurden.

Tags

Bioengineering Molekulare Federkonstante Biomembrankraftsonde dynamische Kraftspektroskopie Stretch-Assay Integrin
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