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Journal / Immunology and Infection / Analyse af SAMHD1-begrænsning ved flowcytometri i …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse af SAMHD1-begrænsning ved flowcytometri i humane myeloide U937-celler
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Analyse af SAMHD1-begrænsning ved flowcytometri i humane myeloide U937-celler

Article DOI: 10.3791/62502-v • 09:43 min • June 13th, 2021
June 13th, 2021 •
1, 2, 1, 3
1Retrovirus-Host Interactions Laboratory, The Francis Crick Institute, 2Retroviral Replication Laboratory, The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine, University of Cambridge

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Beskrevet her er en etableret metode til at bestemme omfanget af HIV-1-begrænsning af det cellulære hæmmende protein SAMHD1. Humane myeloide afstamning U937-celler transduceres med en SAMHD1-ekspressionsvektor, der co-udtrykker YFP, differentieret og derefter udfordret med HIV-RFP. Begrænsningsniveauet bestemmes ved flowcytometrianalyse.

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Immunologi og infektion udgave 172 Human Immunodeficiency Virus Type-1 (HIV-1) SAMHD1 begrænsning myeloide celler flowcytometri retrovirus
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