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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse af T-cellereceptor-induceret calciumtilstrømning i primære murin-T-celler ved fuldspektret flowcytometri
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Analyse af T-cellereceptor-induceret calciumtilstrømning i primære murin-T-celler ved fuldspektret flowcytometri

Article DOI: 10.3791/64526-v • 10:01 min • December 16th, 2022
December 16th, 2022 •
1, 1, 1
1Department of Immunology and Microbiology, University of Colorado-Anschutz School of Medicine

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Calciumtilstrømning, et mål for T-cellesignalering, er en effektiv måde at analysere reaktioner på T-cellereceptorstimulering. Denne protokol til multiplexing af Indo-1 med paneler af antistoffer rettet mod celleoverflademolekyler udnytter de meget fleksible muligheder for fuldspektret flowcytometri.

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Immunologi og infektion nummer 190
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