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JoVE Journal
Immunology and Infection

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全谱流式细胞术分析原代小鼠T细胞受体诱导的钙内流
 
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全谱流式细胞术分析原代小鼠T细胞受体诱导的钙内流

Article DOI: 10.3791/64526-v 10:01 min December 16th, 2022
December 16th, 2022

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Summary

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钙内流是T细胞信号传导的量度,是分析对T细胞受体刺激反应的有效方法。该协议将Indo-1与针对细胞表面分子的抗体组合多重化,利用了全光谱流式细胞术的高度灵活性。

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免疫学与感染,第190期,
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