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JoVE Journal Cancer Research
En etiketfri segmenteringsmetode til intravital billeddannelse af brysttumormikromiljø
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En etiketfri segmenteringsmetode til intravital billeddannelse af brysttumormikromiljø

Article DOI: 10.3791/63413-v • 10:39 min • May 24th, 2022
May 24th, 2022 •
1, 1, 1,2, 1, 1,3
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Department of Pathology, University of Wisconsin-Madison, 3Carbone Cancer Center, University of Wisconsin-Madison

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Den intravitale billeddannelsesmetode, der er beskrevet her, anvender kollagen anden harmonisk generation og endogen fluorescens fra den metaboliske co-faktor NAD (P) H til ikke-invasivt at segmentere et umærket tumormikromiljø i tumor-, stromal- og vaskulære rum til dybdegående analyse af 4D-intravitale billeder.

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Kræftforskning nummer 183
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