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JoVE Journal Cancer Research
En etikettfri segmenteringstilnærming for intravital avbildning av mammary tumormikromiljø
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En etikettfri segmenteringstilnærming for intravital avbildning av mammary tumormikromiljø

Article DOI: 10.3791/63413-v • 10:39 min • May 24th, 2022
May 24th, 2022 •
1, 1, 1,2, 1, 1,3
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Department of Pathology, University of Wisconsin-Madison, 3Carbone Cancer Center, University of Wisconsin-Madison

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Den intravitale avbildningsmetoden som er beskrevet her, bruker kollagen andre harmoniske generasjon og endogen fluorescens fra den metabolske kofaktoren NAD (P)H til ikke-invasivt segmentere et umerket tumormikromiljø i tumor-, stromal- og vaskulære rom for grundig analyse av 4D intravitale bilder.

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