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JoVE Journal Bioengineering
Forberedelse og strukturel evaluering af epitelcellemonolag i en fysiologisk størrelse mikrofluidisk kulturanordning
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Forberedelse og strukturel evaluering af epitelcellemonolag i en fysiologisk størrelse mikrofluidisk kulturanordning

Article DOI: 10.3791/64148-v • 07:38 min • July 1st, 2022
July 1st, 2022 •
1, 1, 1, 1
1Department of Biomedical Engineering, School of Science and Engineering, Tulane University

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Den præsenterede protokol beskriver udviklingen og anvendelsen af en phalloidin-baseret filamentøs actinfarvningsteknik med konfokal laserscanningsmikroskopi (CLSM) til visualisering af klæbende cellelagstruktur i mikrofluidiske dynamiske kulturkanaler og traditionelle statiske kulturkamre med fast brønd. Denne tilgang hjælper med at evaluere cellelagssammenløb, monolagsdannelse og lagtykkelsesensartethed.

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Denne måned i JoVE nummer 185
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