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Journal / Cancer Research / Multiplexed stregkode billedanalyse til…
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Cancer Research

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JoVE Journal Cancer Research
Multiplexed stregkode billedanalyse til immunprofilering og rumlig kortlægning karakterisering i enkeltcelleanalyse af paraffinvævsprøver
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Multiplexed stregkode billedanalyse til immunprofilering og rumlig kortlægning karakterisering i enkeltcelleanalyse af paraffinvævsprøver

Article DOI: 10.3791/64758-v • 08:18 min • April 7th, 2023
April 7th, 2023 •
*1, *1, 1, 1, 1
1Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center
* These authors contributed equally

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Multiplexed stregkode billedanalyse har for nylig forbedret karakteriseringen af tumormikromiljøet, hvilket tillader omfattende undersøgelser af cellesammensætning, funktionel tilstand og celle-celle-interaktioner. Heri beskriver vi en farvnings- og billeddannelsesprotokol ved hjælp af stregkoden af oligonukleotidkonjugerede antistoffer og cyklusbilleddannelse, som muliggør anvendelse af en højdimensionel billedanalyseteknik.

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Kræftforskning nr. 194
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