Shuichi Takayama Department of Biomedical Engineering University of Michigan Biography Publications Institution JoVE Articles Shuichi Takayama has not added a biography. If you are Shuichi Takayama and would like to personalize this page please email our Author Liaison for assistance. Publications Room Temperature Phosphorescence of Metal-free Organic Materials in Amorphous Polymer Matrices Journal of the American Chemical Society. Mar, 2013 | Pubmed ID: 23521108 Microfluidic Oscillators with Widely Tunable Periods Lab on a Chip. Mar, 2013 | Pubmed ID: 23429765 Large-Scale, Ultrapliable, and Free-Standing Nanomembranes Advanced Materials (Deerfield Beach, Fla.). Feb, 2013 | Pubmed ID: 23423854 Localized Micro-scale Disruption of Cells Using Laser-generated Focused Ultrasound Journal of Biophotonics. Feb, 2013 | Pubmed ID: 23420806 Productive Chemical Interaction Between a Bacterial Microcolony Couple is Enhanced by Periodic Relocation Journal of the American Chemical Society. Feb, 2013 | Pubmed ID: 23316688 Super-Resolution Imaging of PDMS Nanochannels by Single-Molecule Micelle-Assisted Blink Microscopy The Journal of Physical Chemistry. B. Jan, 2013 | Pubmed ID: 23256598 Microfluidic Systems: a New Toolbox for Pluripotent Stem Cells Biotechnology Journal. Feb, 2013 | Pubmed ID: 23125055 Analyzing Threshold Pressure Limitations in Microfluidic Transistors for Self-regulated Microfluidic Circuits Applied Physics Letters. Dec, 2012 | Pubmed ID: 23284181 Nanoscale Squeezing in Elastomeric Nanochannels for Single Chromatin Linearization Nano Letters. Dec, 2012 | Pubmed ID: 23186544 Aqueous Two-phase System-derived Biofilms for Bacterial Interaction Studies Biomacromolecules. Sep, 2012 | Pubmed ID: 22793044 Microfluidic Automation Using Elastomeric Valves and Droplets: Reducing Reliance on External Controllers Small (Weinheim an Der Bergstrasse, Germany). Oct, 2012 | Pubmed ID: 22761019 Lab-on-a-chip Biophotonics: Its Application to Assisted Reproductive Technologies Journal of Biophotonics. Jun, 2012 | Pubmed ID: 22700221 Opportunities and Challenges for Use of Tumor Spheroids As Models to Test Drug Delivery and Efficacy Journal of Controlled Release : Official Journal of the Controlled Release Society. Dec, 2012 | Pubmed ID: 22613880 Quantitative Analysis of Molecular Absorption into PDMS Microfluidic Channels Annals of Biomedical Engineering. Sep, 2012 | Pubmed ID: 22484830 Real Time Culture and Analysis of Embryo Metabolism Using a Microfluidic Device with Deformation Based Actuation Lab on a Chip. Jun, 2012 | Pubmed ID: 22402469 Rapid Generation of Multiplexed Cell Cocultures Using Acoustic Droplet Ejection Followed by Aqueous Two-phase Exclusion Patterning Tissue Engineering. Part C, Methods. Sep, 2012 | Pubmed ID: 22356298 Constant Flow-driven Microfluidic Oscillator for Different Duty Cycles Analytical Chemistry. Jan, 2012 | Pubmed ID: 22206453 384 Hanging Drop Arrays Give Excellent Z-factors and Allow Versatile Formation of Co-culture Spheroids Biotechnology and Bioengineering. May, 2012 | Pubmed ID: 22161651 Secreted CXCL12 (SDF-1) Forms Dimers Under Physiological Conditions The Biochemical Journal. Mar, 2012 | Pubmed ID: 22142194 Micro-ring Structures Stabilize Microdroplets to Enable Long Term Spheroid Culture in 384 Hanging Drop Array Plates Biomedical Microdevices. Apr, 2012 | Pubmed ID: 22057945 Rethinking in Vitro Embryo Culture: New Developments in Culture Platforms and Potential to Improve Assisted Reproductive Technologies Biology of Reproduction. Mar, 2012 | Pubmed ID: 21998170 Polymeric Aqueous Biphasic System Rehydration Facilitates High Throughput Cell Exclusion Patterning For Cell Migration Studies Advanced Functional Materials. Aug, 2011 | Pubmed ID: 23519702 Organs-on-a-Chip: A Focus on Compartmentalized Microdevices Annals of Biomedical Engineering. Nov, 2011 | Pubmed ID: 22065201 Receptor Differential Activation and Cooperativity Better Explain Cellular Preference for Different Chemoattractant Gradient Shapes in an EGFR System Integrative Biology : Quantitative Biosciences from Nano to Macro. Oct, 2011 | Pubmed ID: 21918787 Rounded Multi-level Microchannels with Orifices Made in One Exposure Enable Aqueous Two-phase System Droplet Microfluidics Lab on a Chip. Oct, 2011 | Pubmed ID: 21892481 Next-generation Integrated Microfluidic Circuits Lab on a Chip. Sep, 2011 | Pubmed ID: 21799977 Responses of Endothelial Cells to Extremely Slow Flows Biomicrofluidics. Jun, 2011 | Pubmed ID: 21799717 Precisely Targeted Delivery of Cells and Biomolecules Within Microchannels Using Aqueous Two-phase Systems Biomedical Microdevices. Dec, 2011 | Pubmed ID: 21769637 Hi-Fi Transmission of Periodic Signals Amid Cell-to-cell Variability Molecular BioSystems. Jul, 2011 | Pubmed ID: 21559542 Microprinted Feeder Cells Guide Embryonic Stem Cell Fate Biotechnology and Bioengineering. May, 2011 | Pubmed ID: 21538333 Aqueous Biphasic Microprinting Approach to Tissue Engineering Biomicrofluidics. 2011 | Pubmed ID: 21522494 Epithelium Damage and Protection During Reopening of Occluded Airways in a Physiologic Microfluidic Pulmonary Airway Model Biomedical Microdevices. Aug, 2011 | Pubmed ID: 21487664 Artificial Hematopoietic Stem Cell Niche: Bioscaffolds to Microfluidics to Mathematical Simulations Current Topics in Medicinal Chemistry. 2011 | Pubmed ID: 21446910 Flexible Fabrication and Applications of Polymer Nanochannels and Nanoslits Chemical Society Reviews. Jul, 2011 | Pubmed ID: 21442106 Combination of Fluid and Solid Mechanical Stresses Contribute to Cell Death and Detachment in a Microfluidic Alveolar Model Lab on a Chip. Feb, 2011 | Pubmed ID: 21152526 High-density Fabrication of Normally Closed Microfluidic Valves by Patterned Deactivation of Oxidized Polydimethylsiloxane Lab on a Chip. Feb, 2011 | Pubmed ID: 21132212 High-throughput 3D Spheroid Culture and Drug Testing Using a 384 Hanging Drop Array The Analyst. Feb, 2011 | Pubmed ID: 20967331 Phase-locked Signals Elucidate Circuit Architecture of an Oscillatory Pathway PLoS Computational Biology. 2010 | Pubmed ID: 21203481 Multiplexed Spectral Signature Detection for Microfluidic Color-coded Bioparticle Flow Analytical Chemistry. Nov, 2010 | Pubmed ID: 20979407 Microfluidic Platform for Chemotaxis in Gradients Formed by CXCL12 Source-sink Cells Integrative Biology : Quantitative Biosciences from Nano to Macro. Nov, 2010 | Pubmed ID: 20871938 Micropatterning Bacterial Suspensions Using Aqueous Two Phase Systems The Analyst. Nov, 2010 | Pubmed ID: 20838666 Uniform Cell Seeding and Generation of Overlapping Gradient Profiles in a Multiplexed Microchamber Device with Normally-closed Valves Lab on a Chip. Nov, 2010 | Pubmed ID: 20835429 Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures Tissue Engineering. Part C, Methods. Aug, 2010 | Pubmed ID: 20673133 The Art of Reporter Proteins in Science: Past, Present and Future Applications BMB Reports. Jul, 2010 | Pubmed ID: 20663405 Regulating Microenvironmental Stimuli for Stem Cells and Cancer Cells Using Microsystems Integrative Biology : Quantitative Biosciences from Nano to Macro. Jun, 2010 | Pubmed ID: 20535416 Integrated Elastomeric Components for Autonomous Regulation of Sequential and Oscillatory Flow Switching in Microfluidic Devices Nature Physics. Jun, 2010 | Pubmed ID: 20526435 Instantaneous Fabrication of Arrays of Normally Closed, Adjustable, and Reversible Nanochannels by Tunnel Cracking Lab on a Chip. Jun, 2010 | Pubmed ID: 20517560 Simultaneous Fabrication of PDMS Through-holes for Three-dimensional Microfluidic Applications Lab on a Chip. Aug, 2010 | Pubmed ID: 20502832 Guided Corona Generates Wettability Patterns That Selectively Direct Cell Attachment Inside Closed Microchannels Biomedical Microdevices. Oct, 2010 | Pubmed ID: 20495872 Polymeric Aqueous Biphasic Systems for Non-contact Cell Printing on Cells: Engineering Heterocellular Embryonic Stem Cell Niches Advanced Materials (Deerfield Beach, Fla.). Jun, 2010 | Pubmed ID: 20449846 Single Cell Trapping in Larger Microwells Capable of Supporting Cell Spreading and Proliferation Microfluidics and Nanofluidics. Feb, 2010 | Pubmed ID: 20352022 Patterned Electrode-based Amperometric Gas Sensor for Direct Nitric Oxide Detection Within Microfluidic Devices Analytical Chemistry. Apr, 2010 | Pubmed ID: 20329749 Fabrication of Two-layered Channel System with Embedded Electrodes to Measure Resistance Across Epithelial and Endothelial Barriers Analytical Chemistry. Mar, 2010 | Pubmed ID: 20178370 Dynamics of Liquid Plugs of Buffer and Surfactant Solutions in a Micro-engineered Pulmonary Airway Model Langmuir : the ACS Journal of Surfaces and Colloids. Mar, 2010 | Pubmed ID: 20017471 Bioengineered Three-dimensional Physiological Model of Colonic Longitudinal Smooth Muscle in Vitro Tissue Engineering. Part C, Methods. Oct, 2010 | Pubmed ID: 20001822 Patterning Alginate Hydrogels Using Light-directed Release of Caged Calcium in a Microfluidic Device Biomedical Microdevices. Feb, 2010 | Pubmed ID: 19830565 High-speed Tuning of Visible Laser Wavelength Using a Nanoimprinted Grating Optical Tunable Filter Applied Physics Letters. Nov, 2009 | Pubmed ID: 20052314 Microfluidic Hydrodynamic Cellular Patterning for Systematic Formation of Co-culture Spheroids Integrative Biology : Quantitative Biosciences from Nano to Macro. Dec, 2009 | Pubmed ID: 20027373 Microfluidics Cell Culture with Sensing and SqueezeFluidics Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference. 2009 | Pubmed ID: 19964166 Microfluidic Culture of Single Human Embryonic Stem Cell Colonies Lab on a Chip. Jun, 2009 | Pubmed ID: 19495459 Microfluidic Endothelium for Studying the Intravascular Adhesion of Metastatic Breast Cancer Cells PloS One. 2009 | Pubmed ID: 19484126 External Compression-induced Fracture Patterning on the Surface of Poly(dimethylsiloxane) Cubes and Microspheres Langmuir : the ACS Journal of Surfaces and Colloids. Mar, 2009 | Pubmed ID: 19437776 Quantitative Inference of Cellular Parameters from Microfluidic Cell Culture Systems Biotechnology and Bioengineering. Aug, 2009 | Pubmed ID: 19388086 Hard Top Soft Bottom Microfluidic Devices for Cell Culture and Chemical Analysis Analytical Chemistry. May, 2009 | Pubmed ID: 19382754 Microfluidic System for Formation of PC-3 Prostate Cancer Co-culture Spheroids Biomaterials. Jun, 2009 | Pubmed ID: 19304321 A Student Team in a University of Michigan Biomedical Engineering Design Course Constructs a Microfluidic Bioreactor for Studies of Zebrafish Development Zebrafish. Jun, 2009 | Pubmed ID: 19292670 Microfeature Guided Skeletal Muscle Tissue Engineering for Highly Organized 3-dimensional Free-standing Constructs Biomaterials. Feb, 2009 | Pubmed ID: 19064284 The Mechanical Properties of a Surface-modified Layer on Poly(dimethylsiloxane) Journal of Materials Research. Jan, 2008 | Pubmed ID: 19779588 Unsteady Propagation of a Liquid Plug in a Liquid-lined Straight Tube Physics of Fluids (Woodbury, N.Y. : 1994). Jun, 2008 | Pubmed ID: 19547724 Liquid and Surfactant Delivery into Pulmonary Airways Respiratory Physiology & Neurobiology. Nov, 2008 | Pubmed ID: 18585985 Electromechanical Properties of Pressure-actuated Poly(dimethylsiloxane) Microfluidic Push-down Valves Analytical Chemistry. Aug, 2008 | Pubmed ID: 18576665 Individually Programmable Cell Stretching Microwell Arrays Actuated by a Braille Display Biomaterials. Jun, 2008 | Pubmed ID: 18342367 DNA Linearization Through Confinement in Nanofluidic Channels Analytical and Bioanalytical Chemistry. Aug, 2008 | Pubmed ID: 18340435 Reversible On-demand Cell Alignment Using Reconfigurable Microtopography Biomaterials. Apr, 2008 | Pubmed ID: 18192004 Acoustically Detectable Cellular-level Lung Injury Induced by Fluid Mechanical Stresses in Microfluidic Airway Systems Proceedings of the National Academy of Sciences of the United States of America. Nov, 2007 | Pubmed ID: 18006663 Small Volume Low Mechanical Stress Cytometry Using Computer-controlled Braille Display Microfluidics Lab on a Chip. Nov, 2007 | Pubmed ID: 17960277 Assessment of Sperm Chemokinesis with Exposure to Jelly Coats of Sea Urchin Eggs and Resact: a Microfluidic Experiment and Numerical Study The Journal of Experimental Biology. Nov, 2007 | Pubmed ID: 17951422 Efficient Formation of Uniform-sized Embryoid Bodies Using a Compartmentalized Microchannel Device Lab on a Chip. Jun, 2007 | Pubmed ID: 17538720 Tuneable Elastomeric Nanochannels for Nanofluidic Manipulation Nature Materials. Jun, 2007 | Pubmed ID: 17486084 Leakage-free Bonding of Porous Membranes into Layered Microfluidic Array Systems Analytical Chemistry. May, 2007 | Pubmed ID: 17388566 Gravity-driven Microfluidic Particle Sorting Device with Hydrodynamic Separation Amplification Analytical Chemistry. Feb, 2007 | Pubmed ID: 17297936 Characterization and Resolution of Evaporation-mediated Osmolality Shifts That Constrain Microfluidic Cell Culture in Poly(dimethylsiloxane) Devices Analytical Chemistry. Feb, 2007 | Pubmed ID: 17263345 Quantitative Measurement and Control of Oxygen Levels in Microfluidic Poly(dimethylsiloxane) Bioreactors During Cell Culture Biomedical Microdevices. Apr, 2007 | Pubmed ID: 17160707 Optical Imaging in Microfluidic Bioreactors Enables Oxygen Monitoring for Continuous Cell Culture Journal of Biomedical Optics. Sep-Oct, 2006 | Pubmed ID: 17092147 A Surface-modified Sperm Sorting Device with Long-term Stability Biomedical Microdevices. Jun, 2006 | Pubmed ID: 16688569 The Effect of Continuous Wavy Micropatterns on Silicone Substrates on the Alignment of Skeletal Muscle Myoblasts and Myotubes Biomaterials. Aug, 2006 | Pubmed ID: 16650470 Microscale Integrated Sperm Sorter Methods in Molecular Biology (Clifton, N.J.). 2006 | Pubmed ID: 16508075 Handheld Recirculation System and Customized Media for Microfluidic Cell Culture Lab on a Chip. Jan, 2006 | Pubmed ID: 16372083 IVF Within Microfluidic Channels Requires Lower Total Numbers and Lower Concentrations of Sperm Human Reproduction (Oxford, England). Feb, 2006 | Pubmed ID: 16199424 Microfluidic Applications for Andrology Journal of Andrology. Nov-Dec, 2005 | Pubmed ID: 16291957 Computer-controlled Microcirculatory Support System for Endothelial Cell Culture and Shearing Analytical Chemistry. Jul, 2005 | Pubmed ID: 15987102 Fabrication of Reconfigurable Protein Matrices by Cracking Nature Materials. May, 2005 | Pubmed ID: 15834415 Microfluidics for Flow Cytometric Analysis of Cells and Particles Physiological Measurement. Jun, 2005 | Pubmed ID: 15798290 Computerized Microfluidic Cell Culture Using Elastomeric Channels and Braille Displays Proceedings of the National Academy of Sciences of the United States of America. Nov, 2004 | Pubmed ID: 15514025 Arrays of Horizontally-oriented Mini-reservoirs Generate Steady Microfluidic Flows for Continuous Perfusion Cell Culture and Gradient Generation The Analyst. Nov, 2004 | Pubmed ID: 15508030 Fabrication of Microfluidic Mixers and Artificial Vasculatures Using a High-brightness Diode-pumped Nd:YAG Laser Direct Write Method Lab on a Chip. Nov, 2003 | Pubmed ID: 15007466 Reversible Switching of High-speed Air-liquid Two-phase Flows Using Electrowetting-assisted Flow-pattern Change Journal of the American Chemical Society. Dec, 2003 | Pubmed ID: 14640622 Rethinking Gamete/embryo Isolation and Culture with Microfluidics Human Reproduction Update. Sep-Oct, 2003 | Pubmed ID: 14640377 Isolation of Motile Spermatozoa from Semen Samples Using Microfluidics Reproductive Biomedicine Online. Jul-Aug, 2003 | Pubmed ID: 12930579 Passively Driven Integrated Microfluidic System for Separation of Motile Sperm Analytical Chemistry. Apr, 2003 | Pubmed ID: 12705601 Selective Chemical Treatment of Cellular Microdomains Using Multiple Laminar Streams Chemistry & Biology. Feb, 2003 | Pubmed ID: 12618184 Micro- and Nanotechnologies for Studying Cellular Function Current Topics in Medicinal Chemistry. 2003 | Pubmed ID: 12570859 Lateral Propagation of EGF Signaling After Local Stimulation is Dependent on Receptor Density Developmental Cell. Aug, 2002 | Pubmed ID: 12194855 Cell Co-culture Patterning Using Aqueous Two-phase Systems John P. Frampton1, Joshua B. White1, Abin T. Abraham1, Shuichi Takayama1,2 1Department of Biomedical Engineering, University of Michigan, 2Department of Macromolecular Science and Engineering, University of Michigan JoVE 50304 Bio-ingénierie
Cell Co-culture Patterning Using Aqueous Two-phase Systems John P. Frampton1, Joshua B. White1, Abin T. Abraham1, Shuichi Takayama1,2 1Department of Biomedical Engineering, University of Michigan, 2Department of Macromolecular Science and Engineering, University of Michigan JoVE 50304 Bio-ingénierie