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Biology

Published: September 29, 2023 doi: 10.3791/65788

Materials

Name Company Catalog Number Comments
14 mL culture tubes Falcon 352051
15 mL centrifuge tube Falcon 352096
Bacto Agar Baeton,Dickinson and Company 214010
Bacto Typtic Soy Broth Baeton,Dickinson and Company 211825
D-(+)-Glucose Sigma G7021-1KG
D-(+)-Raffinose pentahydrate Sigma 83400-25G
Difco Lactobacilli MRS broth Baeton,Dickinson and Company 288130
EASY-LOCK MICROPR. 1.5 mL (Eppendorf) FL medical 23053
IDEAS Software Amnis/EMD Millipore N/A  Details available at: https://www.merckmillipore.com/INTL/en/20150212_144049?ReferrerURL=https%3A%2F%2Fwww.google.com%2F&bd=1
ImageStream X Mark II Amnis/EMD Millipore N/A  Details available at: https://www.merckmillipore.com/INTL/en/20150121_205948?ReferrerURL=https%3A%2F%2Fwww.google.com%2F
MOPS, 3-(N-morpholino)propanesulfonic acid Fisher bioreagents BP308-500
Potassium phosphate dibasic Fisher Scientific, 174.18 g/mol BP363-1
Potassium phosphate monobasic Sigma, 136.09 g/mol P0662-500G

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References

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  3. Inoue, T., et al. Molecular characterization of low-molecular-weight component protein, Flp, in Actinobacillus actinomycetemcomitans fimbriae. Medical Microbiology and Immunology. 42 (4), 253-258 (1998).
  4. Boddey, J. A., Flegg, C. P., Day, C. J., Beacham, I. R., Peak, I. R. Temperature-regulated microcolony formation by Burkholderia pseudomallei requires pilA and enhances association with cultured human cells. Infection and Immunity. 74 (9), 5374-5381 (2006).
  5. Suissa, R., et al. Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars. Frontiers in Microbiology. 13, 949932 (2022).
  6. Isenring, J., Geirnaert, A., Lacroix, C., Stevens, M. J. A. Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota. BMC Microbiology. 21, 268 (2021).
  7. Kos, B., et al. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. Journal of Applied Microbiology. 94 (6), 981-987 (2003).
  8. Zommiti, M., et al. In vitro assessment of the probiotic properties and bacteriocinogenic potential of Pediococcus pentosaceus MZF16 isolated from artisanal tunisian meat "Dried Ossban". Frontiers in Microbiology. 9, 2607 (2018).
  9. Suissa, R., et al. Metabolic rewiring of the probiotic bacterium rhamnosus GG contributes to cell-wall remodeling and antimicrobials production. bioRxiv. , (2023).
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  12. Sherlock, O., Schembri, M. A., Reisner, A., Klemm, P. Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: cell aggregation and biofilm formation. Journal of Bacteriology. 186 (23), 8058-8065 (2004).
  13. Imaging flow cytometry. Nature Reviews Methods Primers. 2, 87 (2022).
  14. Wang, Y., et al. Metabolism characteristics of lactic acid bacteria and the expanding applications in food industry. Frontiers in Bioengineering and Biotechnology. 9, 612285 (2021).
  15. Turroni, F., et al. Molecular dialogue between the human gut microbiota and the host: a Lactobacillus and Bifidobacterium perspective. Cellular and Molecular Life Sciences. 71, 183-203 (2014).
  16. Zuba-Surma, E. K., Kucia, M., Abdel-Latif, A., Lillard, J. W. Jr, Ratajczak, M. Z. The ImageStream System: a key step to a new era in imaging. Folia Histochemica et Cytobiologica. 45, 279-290 (2007).
  17. Dashkova, V., Malashenkov, D., Poulton, N., Vorobjev, I., Barteneva, N. S. Imaging flow cytometry for phytoplankton analysis. Methods. 112, 188-200 (2017).
  18. Maan, H., Gilhar, O., Porat, Z., Kolodkin-Gal, I. Bacillus subtilis colonization of arabidopsis thaliana roots induces multiple biosynthetic clusters for antibiotic production. Frontiers in Cellular and Infection Microbiology. 11, 722778 (2021).
  19. Maan, H., et al. Imaging flow cytometry reveals a dual role for exopolysaccharides in biofilms: To promote self-adhesion while repelling non-self-community members. Computational and Structural Biotechnology Journal. 20, 15-25 (2022).
  20. Konieczny, M., Rhein, P., Czaczyk, K., Bialas, W., Juzwa, W. Imaging flow cytometry to study biofilm-associated microbial aggregates. Molecules. 26 (23), 7096 (2021).
  21. Niederdorfer, R., Peter, H., Battin, T. J. Attached biofilms and suspended aggregates are distinct microbial lifestyles emanating from differing hydraulics. Nature Microbiology. 1, 16178 (2016).
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Cite this Article

Suissa, R., Hadad, U., Meijler, M.,More

Suissa, R., Hadad, U., Meijler, M., Kolodkin-Gal, I. Imaging Flow Cytometry to Study Microbial Autoaggregation. J. Vis. Exp. (199), e65788, doi:10.3791/65788 (2023).

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