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Measuring the Induced Membrane Voltage with Di-8-ANEPPS
 

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

Article DOI: 10.3791/1659-v • 05:53 min • November 19th, 2009
November 19th, 2009 •
1, 1, 1
1Department of Biomedical Engineering, Faculty of Electrical Engineering, University of Ljubljana

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External electric field induces a voltage on the membrane of a cell, termed the induced membrane voltage (ΔΦ). By using the potentiometric dye di-8-ANEPPS, it is possible to measure the ΔΦ noninvasively. This video shows the protocol for measuring ΔΦ using di-8-ANEPPS.

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Induced Membrane Voltage Di-8-ANEPPS Cell Electric Field Charge Redistribution Cell Membrane Voltage Induced Transmembrane Voltage Induced Transmembrane Potential Difference Delta Phi Resting Voltage Potentiometric Fluorescent Dyes Variations Noninvasively Lipid Bilayer Fluorescence Intensity Protocol Cell Shape Amplitude Spatial Distribution
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