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Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
 

Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy

Article DOI: 10.3791/2069-v • 11:38 min • August 31st, 2010
August 31st, 2010 •
1, 1
1Department of Anesthesiology & Pain Medicine, University of Washington School of Medicine

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Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.

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Cardiac Function Energetics Isolated Mouse Hearts 31P NMR Spectroscopy Bioengineered Mouse Models Cardiovascular Disease Molecular Alterations Signaling Pathway Physiology Biochemical Assessment Metabolites Intact Beating Heart Isolated Heart Perfusions Nuclear Magnetic Resonance (NMR) Spectroscopy Left Ventricular Function Langendorff-mode Perfusions Cardiac Energetics 31P Magnetic Resonance Spectroscopy Phosphocreatine And ATP Levels Physiologic Stressors Pathologic Stressors Ischemia/reperfusion High Workload Challenge Protocols Aortic Banding Cardiac Pathology
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