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Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
 

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Article DOI: 10.3791/2160-v • 11:00 min • September 9th, 2010
September 9th, 2010 •
*1, *1, 1
1Department of Molecular and Cellular Biology, University of Guelph
* These authors contributed equally

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In this video we use an adenovirus carrying the Cre recombinase gene to infect primary mouse embryonic fibroblasts carrying a floxed Rac1 allele.

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Adenovirus-mediated Genetic Removal Signaling Molecules Cultured Primary Mouse Embryonic Fibroblasts Cre-loxP System Conditional Deletion Cre Recombinase Floxed DNA Recombination Event Excision Adenovirus Cre Recombinase Gene Infect Primary Mouse Embryonic Fibroblasts (MEFs) Floxed Rac1 Allele Morphological Changes Actin Cytoskeleton Viral Transduction Cre Expression Stained Using Phalloidin Dye Confocal Laser Scanning Microscopy
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