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In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
 

In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

Article DOI: 10.3791/2249-v • 17:51 min • September 10th, 2010
September 10th, 2010 •
*1,2, *1, 1,2, 1, 1, 1
1Junior Research Group Synaptic Plasticity, Hertie Institute for Clinical Brain Research, University of Tübingen, 2Graduate School of Cellular and Molecular Neuroscience, University of Tübingen
* These authors contributed equally

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This protocol describes a reliable method for anesthetization and imaging of intact Drosophila melanogaster larvae. We have utilized the volatile anesthetic desflurane to allow for repetitive imaging at sub-cellular resolution and re-identification of structures for up to a few days1.

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In Vivo Imaging Drosophila Larvae Sub-cellular Resolution Optical Imaging Genetically Encoded Fluorophores Genetic Tools Transgenic Animal Lines Anesthetize Larvae Desflurane Synaptic Populations High Degree Of Anesthetization Survival Rate Time-points Proteins Dynamics Glutamate Receptor Subunit GluR-IIA Endogenous Promoter Exon Trap Line FasII-GFP
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