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Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
 

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Article DOI: 10.3791/1510-v • 11:51 min • August 20th, 2009
August 20th, 2009 •
1, 2, 3, 3, 2, 1
1Center for Systems Biology, Massachusetts General Hospital, 2Institute for Biological and Medical Imaging (IBMI), Technical University of Munich and Helmholtz Center Munich, 3Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute

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Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.

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Mesoscopic Fluorescence Tomography In-vivo Imaging Developing Drosophila Optical Interrogation Molecular Changes Functional Changes Optical Imaging Methods Penetration Limits Optical Microscopy Diffusion-imposed Limits Optical Macroscopy Model Organisms Insects Animal Embryos Small Animal Extremities Nanometer-resolution Optical Imaging Imaging Penetration Depth Photon Scattering Tissues Entire Embryos Imaging Chemical Treatment Scattering Clearance Post-mortem Imaging Two-photon Microscopy Confocal Microscopy Developmental Biology Drug Discovery
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