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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
 

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Article DOI: 10.3791/2785-v • 07:35 min • June 2nd, 2011
June 2nd, 2011 •
*1, *2, 2, 1,2,3
1Molecular and Cell Biology Program, Ohio University, 2Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering, Russ College of Engineering and Technology, Ohio University
* These authors contributed equally

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Dendritic cells uptake antigens and migrate towards immune organs to present processed antigens to T cells. Qdot nanocrystal labeling provides a long-lasting and stable fluorescent signal. This allows tracking of dendritic cells to different organs by fluorescent microscopy.

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Generation Labeling Murine Bone Marrow-derived Dendritic Cells Qdot Nanocrystals Tracking Studies Antigen Presenting Cells Peripheral Tissues Immunological Organs Major Histocompatibility Molecules Migratory Capabilities Fluorescent Dyes Qdot Fluorescent Nanocrystals Stable Fluorescence Recovered Tissues Cell Culture Differentiation In Vitro Incubation Fluorescent Microscopy Experimental Animals Mature Cells
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