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Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
 

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Article DOI: 10.3791/3163-v • 11:48 min • April 7th, 2012
April 7th, 2012 •
*1, *2, 1, 3, 2,3,4, 1,2
1Biosphere Oriented Biology Research Unit, RIKEN Advanced Science Institute, 2Graduate School of Nanobioscience, Yokohama City University, 3Advanced NMR Metabomics Research Team, RIKEN Plant Science Center, 4Graduate School of Bioagricultural Science, Nagoya University
* These authors contributed equally

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résumé
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A method for metabolite extraction from microbial planktonic communities is presented. Whole community sampling is achieved by filtration onto specially prepared filters. After lyophilization, aqueous-soluble metabolites are extracted. This approach allows for application of environmental metabolomics to trans-omics investigations of natural or experimental microbial communities.

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Concentration Metabolites Low-density Planktonic Communities Environmental Metabolomics Nuclear Magnetic Resonance Spectroscopy NMR Spectroscopy Gas Chromatography-mass Spectrometry GC-MS Untargeted Analyses Structural Information Quantitative Analysis Statistical Analysis Metabolite Spectra Databases Omics Levels Transcriptomics Genomics Physiological Responses Taxa Sensitivity Natural Microbial Systems
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