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Immunology and Infection

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JoVE Journal Immunology and Infection
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
 

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Article DOI: 10.3791/3645-v • 09:47 min • March 1st, 2012
March 1st, 2012 •
1, 2, 2, 2, 1
1Department of Plant Pathology and Microbiology, University of California, Riverside, 2Department of Bioengineering, University of California, Riverside

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résumé
Automatic Translation

A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.

Tags

Virus Assembly In Vivo In Vitro Encapsidation Positive-sense RNA Genomes Infectious Virions Mechanism Regulating Virus Assembly Curbing Virus Spread Disease Control Macromolecular Interactions Subcellular Compartmentalization Biomedical Imaging Therapeutic Applications Non-enveloped Plant Viruses Brome Mosaic Virus (BMV) Genome Packaging Nicotiana Benthamiana Plants Agroinfiltration
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