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Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)
 

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

Article DOI: 10.3791/3875-v • 14:15 min • June 18th, 2012
June 18th, 2012 •
1, 1, 1, 1
1Department of Molecular Medicine, University of South Florida Morsani College of Medicine

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This is a rapid and comprehensive method of immunophenotyping Myeloid Derived Suppressor Cells (MDSC) and enriching Gr-1+ leukocytes from mouse spleens. This method uses flow cytometry and AutoMACS Cell Sorting to enrich for viable Gr-1+ leukocytes prior to FACS sorting of MDSC for use in vivo and in vitro assays.

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Myeloid Derived Suppressor Cells (MDSC) Naive Mice Pancreatic Tumor-bearing Mice Flow Cytometry Automated Magnetic Activated Cell Sorting (AutoMACS) Heterogeneous Population Immature Macrophages Dendritic Cells Granulocytes Lymphoid Organs Parasitic Infection Inflammation Traumatic Stress Graft-versus-host Disease Diabetes Cancer Mac-1 (CD11b) Gr-1 (Ly6G And Ly6C) Tumor-bearing Hosts Anti-tumor Immune Responses Subpopulations Of MDSC Molecular Mechanisms Of Suppression Ghansah Group Murine Pancreatic Cancer Model Homeostasis Suppressive Function Lymphoid Compartments Gr-1+ Leukocytes
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