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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
 

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

Article DOI: 10.3791/4227-v • 14:29 min • October 1st, 2012
October 1st, 2012 •
1, 1, 2, 1
1Department of Immunology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

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résumé
Automatic Translation

Methods for purifying the cholesterol binding toxin streptolysin O from recombinant E. coli and visualization of toxin binding to live eukaryotic cells are described. Localized delivery of toxin induces rapid and complex changes in targeted cells revealing novel aspects of toxin biology.

Tags

Visualization Bacterial Toxin Live Cell Fluorescence Microscopy Membrane Pores Leakage Cellular Contents Influx Toxin Exposure Cell Type Inflammatory Responses Immune Cells Macrophages Pro-inflammatory Cytokines Gram Positive Bacteria Virulence Mechanisms Morphologic Changes Plasma Membrane Cholesterol-enriched Surface Protrusions Extracellular Space Cellular Defense Mechanism EM (electron Microscopy) Chemical Fixation Immune Cells Macrophages Inflammation Membrane Vesicles IL-1 Family Cytokines
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