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RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
 

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Article DOI: 10.3791/4393-v • 18:30 min • February 13th, 2013
February 13th, 2013 •
1, 1, 1, 1, 1, 1, 1
1Children's Mercy Hospital and Clinics, School of Medicine, University of Missouri-Kansas City

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This protocol presents a complete and detailed procedure to apply RNA-seq, a powerful next-generation DNA sequencing technology, to profile transcriptomes in human pulmonary microvascular endothelial cells with or without thrombin treatment. This protocol is generalizable to various cells or tissues affected by different reagents or disease states.

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RNA-seq Analysis Transcriptomes Thrombin-treated Control Human Pulmonary Microvascular Endothelial Cells Gene Expression MRNA Levels External Signals Drug Treatment Healthy State Diseased State Next-generation DNA Sequencing Technology RNA-sequencing Catalog Transcripts Transcriptional Structure Expressed Genes Changing Expression Levels Cell/tissue/organism Complete Transcriptome Profiling Digital Type Datum Copy Number Of Genomic Sequence Protocol Human Pulmonary Microvascular Endothelial Cells With Thrombin Treatment
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