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Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
 

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

Article DOI: 10.3791/64885-v • 05:26 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 2, 2,3, 2,3, 1, 4, 4,5, 1,2, 2,3, 1, 6,7, 1, 1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS

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Although challenging, the isolation of pulmonary endothelial cells is essential for studies on lung inflammation. The present protocol describes a procedure for the high-yield, high-purity isolation of macrovascular and microvascular endothelial cells.

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Keywords: Endothelial Cells Lung Tissue Sample Blood Vessels Purification Isolation Microvascular Macrovascular Collagenase Cell Strainer Centrifugation Cell Culture
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