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Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain
 

Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain

Article DOI: 10.3791/50239-v • 11:00 min • May 19th, 2013
May 19th, 2013 •
*1, *1, *1, 1, 1
1Molecular, Cellular and Developmental Biology, University of Michigan
* These authors contributed equally

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résumé
Automatic Translation

A protocol for cell cycle analysis of live Drosophila tissues using the Attune Acoustic Focusing Cytometer is described. This protocol simultaneously provides information about relative cell size, cell number, DNA content and cell type via lineage tracing or tissue specific expression of fluorescent proteins in vivo.

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Live Cell Cycle Analysis Drosophila Tissues Attune Acoustic Focusing Cytometer Vybrant DyeCycle Violet DNA Stain Flow Cytometry DNA Content Cell Cycle Phases Genetic Studies Cell Number Cell Size Cell Cycle Phasing Cell Permeable Dye Hoechst 33342 UV Laser Live-cell Method Violet 405nm Laser Protocol Cell Type Relative Cell Size Cell Number Information
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