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Immunology and Infection

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JoVE Journal Immunology and Infection
Assays for the Identification of Novel Antivirals against Bluetongue Virus
 

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Article DOI: 10.3791/50820-v • 12:02 min • October 11th, 2013
October 11th, 2013 •
1, 2, 1
1Division of Infectious Diseases, Department of Medicine, University of Alabama at Birmingham, 2Molette Laboratory for Drug Discovery, Department of Chemistry and Biochemistry, Auburn University

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Three assays, including the cytopathic effect (CPE)-based assay, dose-response assay and Time-of-Addition (ToA) assay have been developed, optimized, validated and utilized to identify novel antivirals against Bluetongue virus (BTV), as well as to determine the possible Mechanism-of-Action (MoA) for newly identified antivirals.

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Antiviral Assays BTV Antivirals Cytopathic Effect (CPE) Assay Dose-response Assay Mechanism Of Action (ToA) Assay Antiviral Screening Viral Lifecycle Cell Culture System
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