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JoVE Journal Bioengineering
Quantitative Optical Microscopy
 

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Article DOI: 10.3791/50988-v • 14:09 min • April 7th, 2014
April 7th, 2014 •
1,2, 1, 1,3
1Department of Biomedical Engineering, Oregon Health & Science University, School of Medicine, 2Department of Dermatology, Oregon Health & Science University, School of Medicine, 3Department of Cell & Developmental Biology, Division of Hematology & Medical Oncology, Knight Cancer Institute, Oregon Health & Science University, School of Medicine

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résumé
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We describe the use of a standard optical microscope to perform quantitative measurements of cellular mass, volume, and density through a combination of bright field and differential interference contrast imagery.

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Keywords: Quantitative Optical Microscopy Cellular Biophysical Features Bright Field Differential Interference Contrast Noninterferometric Quantitative Phase Microscopy NIQPM Hilbert Transform Differential Interference Contrast Microscopy HTDIC Paraxial Approximation Low Numerical Aperture Weak Scattering Weak Absorption Volumetric Information High NA Illumination Edge Detection Diffraction Effects
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