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JoVE Journal Bioengineering
FIBS-aktiverede Noninvasiv stofskifteprofil
 
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FIBS-aktiverede Noninvasiv stofskifteprofil

Article DOI: 10.3791/51200-v • 09:21 min • February 3rd, 2014
February 3rd, 2014 •
1,2, 2, 2, 1
1Centre for Process Systems Engineering, Department of Chemical Engineering and Chemical Technology, Imperial College London, 2Centre for Synthetic Biology and Innovation, Division of Molecular Biosciences, Imperial College London

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En beskrivelse af, hvordan du kalibrerer Förster Resonance Energy Transfer integrerede biologiske sensorer (FIBS) til in situ metabolisk profilering præsenteres. FIBS kan anvendes til at måle intracellulære niveauer af metabolitter noninvasively medvirken i udviklingen af ​​metaboliske modeller og high throughput screening af biologisk proces betingelser.

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Bioengineering metabolit overvågning, pattedyrcellekultur bioproces teknik medium formulering
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