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Journal / Neuroscience / Neonatal Pial Surface Electroporation
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JoVE Journal Neuroscience
Neonatal Pial Surface Electroporation
 

Neonatal Pial Surface Electroporation

Article DOI: 10.3791/51319-v • 06:22 min • May 7th, 2014
May 7th, 2014 •
1, 2, *1, *2
1Department of Neurosurgery, Regenerative Medicine Institute, Cedars-Sinai Medical Center, 2Department of Biomedical Sciences, Regenerative Medicine Institute, Cedars-Sinai Medical Center
* These authors contributed equally

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résumé
Automatic Translation

The pial surface is a unique progenitor zone in the CNS that is receiving increasing attention. Herein, we detail a method for rapid genetic manipulation of this progenitor zone using a modified electroporation method. This procedure can be used for cellular and molecular investigations of cell lineages and signaling pathways involved in cell differentiation and to elucidate the fate and properties of daughter cells.

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Keywords: Pial Surface Electroporation Neonatal Progenitor Neural Stem Cell Genetic Manipulation Gliogenesis Neurogenesis Embryonic Postnatal
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