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Developmental Biology

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JoVE Journal Developmental Biology
Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
 

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models

Article DOI: 10.3791/51983-v • 12:01 min • January 12th, 2015
January 12th, 2015 •
1, 1, 1, 1
1Department of Cellular and Molecular Medicine, University of Ottawa

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Neuron migration is regulated by numerous cell autonomous and non-cell autonomous factors. This protocol shows how in utero electroporation can be used to determine whether a phenotype in a transgenic mouse is due to disruption of a cell intrinsic mechanism or impairment of interaction between the neuron and its environment.

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Protein Deletion In Utero Electroporation Transgenic Models Neuronal Migration Cre-Lox System Phenotypes Cell Autonomous Mechanism Non-cell Autonomous Mechanism In Vivo Context Small Subset Of Cells Developing Cortex Brain Region Dorsal Telencephalon Medial Telencephalon Hippocampus Ganglionic Eminence Radial Migration Floxed Transgenic Mouse Line
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