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JoVE Journal
Immunology and Infection

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Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
 

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

Article DOI: 10.3791/52199-v 09:42 min December 23rd, 2014
December 23rd, 2014

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résumé

Periodic acid Schiff staining is a technique that visualizes the polysaccharide content of tissues. This article demonstrates periodic acid Schiff staining protocol adapted for use on peripheral blood mononuclear cells purified from human venous blood. Such samples are enriched for lymphocytes and other white blood cells of the immune system.

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Keywords: PAS Staining Glycogen Peripheral Blood Mononuclear Cells Immunohistochemistry Lymphocytes Glycoproteins Glycolipids Muscle Cells Immune System Energy Source Amylase Enzymatic Digestion
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