Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolating Intestinal Stem Cells from Adult…
JoVE Journal
Biology

Ce contenu est en libre accès.

JoVE Journal Biology
Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
 

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Article DOI: 10.3791/52223-v • 10:57 min • December 16th, 2014
December 16th, 2014 •
*1, *2, 1
1Institut für Biochemie und Molekulare Biologie, Universität Ulm, 2Institut für Immunologie, Universitätsklinikum Ulm
* These authors contributed equally

Chapitres

résumé
Automatic Translation

Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. The method described here presents a simple and robust approach to enrich for and isolate Drosophila intestinal stem cells and the enteroblast progenitor cells by FACS at any time point during aging.

Tags

Intestinal Stem Cells Adult Drosophila Midguts FACS Stem Cell Behavior Aging Tissue Homeostasis Molecular Mechanisms Intestinal Epithelium Molecular Markers Dissociation Of Midgut Tissue Living Cells Distinct Populations Esg-Gal4 UAS-GFP Fly Line Enteroblast Progenitor Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter