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JoVE Journal Immunology and Infection
Modeling Lifecycle af Ebola virus Under biosikkerhedsniveau 2 Betingelser med virus-lignende partikler indeholdende Tetracistronic minigenomer
 
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Modeling Lifecycle af Ebola virus Under biosikkerhedsniveau 2 Betingelser med virus-lignende partikler indeholdende Tetracistronic minigenomer

Article DOI: 10.3791/52381-v • 10:11 min • September 27th, 2014
September 27th, 2014 •
1, 1, 2, 1
1Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

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Arbejde med smitsomme Ebola-virus er begrænset til biosikkerhed niveau 4 laboratorier. Tetracistronic minigenomplasmiderne-holdige replikation og transkription-kompetent viruslignende partikler (trVLPs) udgør et livscyklus modellering system, der giver os mulighed for sikkert at modellere flere smitsomme cyklusser under biosikkerhed niveau 2 betingelser, bygger udelukkende på Ebola-virus komponenter.

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Smitsomme sygdomme blødende feber viral Mononegavirales infektioner ebola-virus Filovirus livscyklus modelsystem minigenomplasmiderne omvendt genetik viruslignende partikler replikation transskription spirende morfogenese indgang
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