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JoVE Journal Immunology and Infection
Tilpasning af gastrointestinale organoider til patogeninfektion og enkeltcellesekvens under biosikkerhedsniveau 3 (BSL-3) Betingelser
 
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Tilpasning af gastrointestinale organoider til patogeninfektion og enkeltcellesekvens under biosikkerhedsniveau 3 (BSL-3) Betingelser

Article DOI: 10.3791/62857-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2, 2,3,4
1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, 3Department of Infectious Diseases, Virology, Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)

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Denne protokol beskriver, hvordan man inficerer menneskelige intestinale organoider fra enten deres apikale eller basolaterale side for at karakterisere værts /patogeninteraktioner på enkeltcelleniveau ved hjælp af encellet RNA-sekventering (scRNAseq) teknologi.

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Immunologi og infektion Udgave 175 humane intestinale organoider encellet RNA sekventering biosikkerhedsniveau 3 organoidinfektion enteriske patogener apikale og basolaterale infektioner
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