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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
 

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

Article DOI: 10.3791/52437-v • 11:09 min • September 11th, 2016
September 11th, 2016 •
1, 1
1National Exposure Research Laboratory, U.S. Environmental Protection Agency

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Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.

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EPA Method 1615 Enterovirus Norovirus Water Culture RT-qPCR Infectious Virus Particles Alkaline-based Extract Solution Electro-positive Filter Virus Concentration Electro-positive Cartridge Filter PH Reduction Flock Formation Centrifugation Sodium Phosphate Buffer Filter Sterilization Buffalo Green Monkey Kidney Cells Cytopathic Effect (CPE) Virus Replication Cell-associated Virus Particles Most Probable Number (MPN) Liter Sample Analysis
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