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Immunology and Infection

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JoVE Journal Immunology and Infection
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
 

Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

Article DOI: 10.3791/52597-v • 09:39 min • December 26th, 2014
December 26th, 2014 •
*1,2,3, *1,2, 1,2, 1,2
1Molecular Pathology, Cancer Center and Center for Regenerative Medicine, Massachusetts General Hospital, 2Harvard Stem Cell Institute, 3GABBA - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto
* These authors contributed equally

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Here, we present a protocol for cell transplantation of zebrafish skeletal muscle and embryonal rhabdomyosarcoma (ERMS) into adult immune compromised rag2E450fs homozygous mutant zebrafish. This protocol allows for the efficient analysis of regeneration and malignant transformation of muscle cells.

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Muscle Cell Transplantation Immune Compromised Zebrafish Allograft Cell Transplantation Stem Cell Function Regeneration Cancer Skeletal Muscle Transplantation Embryonal Rhabdomyosarcoma Pediatric Sarcoma Adult Rag2E450fs Homozygous Mutant Zebrafish T Cells B Cell Function Fluorescently Labeled Muscle Cell Preparations &945;-actin-RFP Transgenic Zebrafish Engraftment Dorsal Musculature Rag2 Homozygous Mutant Fish Fluorescent-transgenic ERMS AB-strain Myf5-GFP; Mylpfa-mCherry Double Transgenic Animals
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