Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Environment / Automated Gel Size Selection to Improve the…
JoVE Journal
Environment

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Environment
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
 

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Article DOI: 10.3791/52685-v • 13:26 min • April 17th, 2015
April 17th, 2015 •
1, 2, 2, 3, 1,3, 1,3, 1,3
1Department of Pathology and Laboratory Medicine, Faculty of Medicine, The University of British Columbia, 2Coastal Genomics, 3British Columbia Public Health Microbiology and Reference Laboratory

Chapitres

résumé
Automatic Translation

This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.

Tags

Keyword Extraction: Next-generation Sequencing Environmental Water Samples DNA Library Preparation Gel Size Selection Automated Electrophoresis Ranger Technology Read Length Read Quality
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter