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JoVE Journal Environment
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
 

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Article DOI: 10.3791/54741-v • 08:04 min • November 25th, 2016
November 25th, 2016 •
1, 2, 3, 4, 4, 2, 1, 5
1Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, 2Graduate School of Human Development and Environment, Kobe University, 3Faculty of Science and Technology, Ryukoku University, 4Okinawa Churashima Research Center, 5Graduate School of Simulation Studies, University of Hyogo

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résumé
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We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.

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Keywords: Filter Cartridge Water Filtration Environmental DNA Metabarcoding Biodiversity Monitoring Community Ecology Vacuum Tubing Aspirator Pump Manifold Luer Fitting Vacuum Connector Plastic Bag Silicone Stopper Book Bottle DNA Extraction
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