Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / A Novel Culture Model for Human Pluripotent Stem…
JoVE Journal
Developmental Biology

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Developmental Biology
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
 

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Article DOI: 10.3791/53204-v • 07:33 min • August 3rd, 2015
August 3rd, 2015 •
1, 1
1Department of Biomedical Science, Graduate School of Medicine, Korea University

Chapitres

résumé
Automatic Translation

This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata.

Tags

Pluripotent Stem Cells Feeder-free Culture Placenta-conditioned Media Gelatin Basic Fibroblast Growth Factor Matrigel Laminin Humanized Culture Contamination Risk Mass Production
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter