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JoVE Journal Developmental Biology
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
 

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Article DOI: 10.3791/53204-v • 07:33 min • August 3rd, 2015
August 3rd, 2015 •
1, 1
1Department of Biomedical Science, Graduate School of Medicine, Korea University

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résumé
Automatic Translation

This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata.

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Human Pluripotent Stem Cells Propagation Gelatin Placenta-conditioned Media Feeder-free Culture Exogenous Substrates Basic Fibroblast Growth Factor HPSC-sustaining Factor Culture Media Synthetic Substrata Matrigel Laminin Humanized Culture Conditions HPSC Contamination Risks Animal Products Virus Contamination Unknown Proteins Mass Production Gelatin Coating Ex Vivo HPSC Maintenance
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